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When a control agent targets the metabolic processes of microbial cells, active younger cells typically die more rapidly than older cells.

A) True
B) False

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Placing organisms at 4oC is ______.


A) bactericidal
B) bacteriostatic
C) decontamination
D) sterilization
E) germicidal

F) A) and E)
G) A) and D)

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Physical agents for controlling microbial growth include all of the following except ______.


A) ultraviolet radiation
B) boiling water
C) pasteurization
D) hydrogen peroxide

E) B) and C)
F) C) and D)

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A method for sterilizing milk, called _____ treatment, uses 134°C for 1 to 2 seconds.


A) pasteurization
B) batch pasteurization
C) flash pasteurization
D) ultra high temperature
E) tyndallization

F) D) and E)
G) B) and D)

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Removal of moisture by dehydration is called ______.


A) flash freeze
B) pasteurization
C) desiccation
D) lyophilization
E) sterilization

F) B) and C)
G) A) and C)

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Some microbial control agents are able to _____ cell proteins by breaking bonds that maintain the native state, the three-dimensional configuration of the proteins.


A) mutate
B) bind
C) dissolve
D) denature

E) None of the above
F) All of the above

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In lab, inoculating loops are sterilized using ______.


A) chemicals
B) incineration
C) filtration
D) moist heat
E) gas sterilization

F) B) and D)
G) B) and C)

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Microbial death occurs when there is


A) no movement.
B) no reproduction.
C) a change in appearance.
D) a decrease in size.
E) All of the choices occur.

F) C) and D)
G) B) and E)

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The use of a physical or chemical process to destroy vegetative pathogens on inanimate objects is ______.


A) disinfection
B) sterilization
C) antisepsis
D) sanitization
E) degermation

F) A) and B)
G) A) and C)

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Alcohols


A) denature proteins when in a 50-95% alcohol-water solution.
B) disinfect items when soaking method is utilized.
C) are skin degerming agents.
D) at 50% or higher concentrations dissolve cell membrane lipids.
E) All of the choices are correct.

F) B) and C)
G) B) and D)

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_____ is a control method that removes microorganisms rather than inhibiting or killing them.


A) Disinfection
B) Filtration
C) Boiling
D) Radiation
E) Sterilization

F) None of the above
G) C) and D)

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Scrubbing or immersing the skin in chemicals to reduce the numbers of microbes on the skin is ______.


A) disinfection
B) sterilization
C) sanitization
D) degermation

E) A) and C)
F) All of the above

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Which control method would not be a suitable choice for killing Mycobacterium in a capped culture tube?


A) Ultraviolet (germicidal) light
B) Gamma rays
C) 121°C at 15 psi for 15 minutes
D) 160°C for 2 hours
E) All of the choices are correct.

F) A) and B)
G) None of the above

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Electrons are ejected from atoms in cells when organisms are exposed to ______.


A) desiccation
B) ultraviolet light
C) ethyl alcohol
D) hydrogen peroxide
E) gamma rays and X rays

F) C) and E)
G) B) and D)

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Your friend Joe recently visited the doctor for steroid injections into his knee to reduce swelling due to a previous knee injury.Within a couple of days, he had an infection caused by Pseudomonas, a very pathogenic and drug resistant bacterium.Luckily, Joe went to the doctor immediately and received antibiotics.The doctor tells him that the same problem has occurred to many other people across the United States.Predict the most likely cause of this situation.


A) The steroid was contaminated at the production plant, so all batches of that drug made at that plant were contaminated with the bacterium.
B) The bacterium is normally on the skin of people, so can easily access the patient's blood during the needle stick.
C) The staff giving the steroid injections did not use proper aseptic technique.
D) The needles were re-used and already contaminated before being used on the patients.

E) B) and D)
F) A) and D)

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The minimum sterilizing conditions in a steam autoclave are


A) 121°C at 15 psi for 15 minutes.
B) 63°C for 30 minutes.
C) 100°C for 30 minutes.
D) 160°C for 2 hours.
E) 71.6°C for 15 seconds.

F) B) and E)
G) All of the above

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The compound that is an organic base containing chlorine and two phenolic rings, and is used increasingly for wound degerming, neonatal washes, hand scrubbing, and prepping surgical skin sites is ______.


A) a quaternary ammonium compound
B) carbolic acid
C) triclosan
D) formalin
E) chlorhexidine

F) A) and B)
G) B) and C)

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Which antimicrobial method does not sterilize?


A) Ionizing radiation
B) Ethylene oxide gas
C) Autoclave
D) Pasteurization

E) A) and C)
F) All of the above

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Which of the following does not affect microbial nucleic acids?


A) Moist heat
B) Ultraviolet light
C) X rays
D) Ethylene dioxide
E) Formaldehyde

F) C) and E)
G) B) and E)

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Vials of microorganisms that undergo the freeze-drying process, called _____, will remain preserved and viable for years.


A) desiccation
B) flash freeze
C) lyophilization
D) pasteurization
E) sterilization

F) A) and B)
G) B) and E)

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